The most ancient Cambrian-like CID/NCBD complex lacked one helix and several interdomain interactions, resulting in a larger solvent-accessible surface area. The most ancient low-affinity “Cambrian-like” CID/NCBD complex contained less secondary structure and was more dynamic than the complexes from an evolutionarily younger “Ordovician-Silurian” fish ancestor (ca. We have used nuclear magnetic resonance spectroscopy to investigate the changes in structure and dynamics during the evolution of a protein-protein interaction involving the intrinsically disordered CREBBP (CREB-binding protein) interaction domain (CID) and nuclear coactivator binding domain (NCBD) from the transcriptional coregulators NCOA (nuclear receptor coactivator) and CREBBP/p300, respectively. However, the molecular details of the evolution of new protein-protein interactions are poorly understood. In every established species, protein-protein interactions have evolved such that they are fit for purpose. Urea and temperature denaturation experiments and Coulomb surfaces of the ancestral and extant CID/NCBD complexes. Restraints used for structure calculations and refinement.įig. CONSPIRE 2018 FREEHSQC correlation spectra for bound and free CID and NCBD domains and the number of restraints in the structure determination as a function of amino acid residue.įig. Frustration in the CID/NCBD complexes without electrostatics.įig. Backbone subnanosecond motions and Δ C p for the three CID/NCBD complexes.įig. 15N relaxation data for the bound CID and NCBD domains and amide secondary chemical shifts of the complexes.įig. Strips from -NOESY -HSQC spectra showing resonances emanating from residues 10 of the CID domains.įig. Strips from -NOESY-HSQC spectra showing resonances emanating from residues 10 of the respective CID domains.įig. Structural changes taking place and chemical shift restraints.įig. Supplementary material for this article is available at įig.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |